DDB2 Antikörper (C-Term)

Produktnummer ABIN117955

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DDB2 in WB, IP und EIA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.Es wurde in 1 Publikation zitiert.

Kurzübersicht für DDB2 Antikörper (C-Term) (ABIN117955)

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-DDB2 Antikörper

Bindungsspezifität: AA 419-427, C-Term

Sequenz: 1 mapkkrpetq ktseivlrpr nkrsrsplel epeakklcak gsgpsrrcds dclwvglagp 61 qilppcrsiv rtlhqhklgr aswpsvqqgl qqsflhtlds yrilqkaapf drratslawh 121 pthpstvavg skggdimlwn fgikdkptfi kgigaggsit glkfnplntn qfyassmegt 181 trlqdfkgni lrvfassdti niwfcsldvs assrmvvtgd nvgnvillnm dgkelwnlrm 241 hkkkvthval npccdwflat asvdqtvkiw dlrqvrgkas flyslphrhp vnaacfspdg 301 arllttdqks eirvysasqw dcplgliphp hrhfqhltpi kaawhprynl ivvgrypdpn 361 fksctpyelr tidvfdgnsg kmmcqlydpe ssgisslnef npmgdtlasa mgyhiliwsq 421 eeartrk

Spezifität: This antibody reacts with DDB2.

Aufreinigung: Processed by delipidation and defibrination followed by sterile filtration

Immunogen: Synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH Genename: DDB2

Anwendungsinformationen

Applikationshinweise: Western blot (1/500-1/1,000 - a 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control). ELISA (1/2,000-1/10,000). Immunoprecipitation (The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using 293T, and others). Coimmunoprecipitation of relatedproteins has not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85.0 mg/mL (by refractometry)

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-DDB2 Antikörper (ABIN117955)

Luijsterburg, Goedhart, Moser, Kool, Geverts, Houtsmuller, Mullenders, Vermeulen, van Driel: "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2706-16, (2007) (PubMed).

Details zu DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Andere Bezeichnung: DDB2

Hintergrund: The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.Synonyms: DDB p48 subunit, DDBb, DNA damage-binding protein 2, Damage-specific DNA-binding protein 2, UV-DDB2, UV-damaged DNA-binding protein 2

Gen-ID: 1643

NCBI Accession: NP_000098

UniProt: Q92466

Pathways: DNA Reparatur