This antibody conjugate is suitable for intracellular staining of cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.
Probenmenge
20 μL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Fornasa, Groyer, Clement, Dimitrov, Compain, Gaston, Varthaman, Khallou-Laschet, Newman, Graff-Dubois, Nicoletti, Caligiuri: "TCR stimulation drives cleavage and shedding of the ITIM receptor CD31." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 10, pp. 5485-92, (2010) (PubMed).
MacGillivray, Herrera-Abreu, Chow, Shek, Wang, Vachon, Feng, Siminovitch, McCulloch, Downey: "The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27190-8, (2003) (PubMed).
Lu, Gong, Bar-Sagi, Cole: "Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling." in: Molecular cell, Vol. 8, Issue 4, pp. 759-69, (2001) (PubMed).
Bennett, Tang, Sugimoto, Walsh, Neel: "Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 7335-9, (1994) (PubMed).
Ahmad, Banville, Zhao, Fischer, Shen: "A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 6, pp. 2197-201, (1993) (PubMed).
Target
PTPN11
(Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
BPTP3 antikoerper, CFC antikoerper, NS1 antikoerper, PTP-1D antikoerper, PTP2C antikoerper, SH-PTP2 antikoerper, SH-PTP3 antikoerper, SHP2 antikoerper, 2700084A17Rik antikoerper, AW536184 antikoerper, PTP1D antikoerper, SAP-2 antikoerper, SHP-2 antikoerper, Shp2 antikoerper, Syp antikoerper, SYP antikoerper, bptp3 antikoerper, cfc antikoerper, ns1 antikoerper, ptp-2 antikoerper, ptp2c antikoerper, ptpn11 antikoerper, ptpn11-a antikoerper, ptpn11-b antikoerper, shp-2 antikoerper, shp2 antikoerper, fa14b09 antikoerper, wu:fa14b09 antikoerper, wu:fi24f03 antikoerper, zgc:55388 antikoerper, zgc:63553 antikoerper, protein tyrosine phosphatase, non-receptor type 11 antikoerper, protein tyrosine phosphatase, non-receptor type 11 S homeolog antikoerper, protein tyrosine phosphatase, non-receptor type 11, a antikoerper, protein tyrosine phosphatase, non-receptor type 11, b antikoerper, PTPN11 antikoerper, Ptpn11 antikoerper, ptpn11.S antikoerper, ptpn11a antikoerper, ptpn11b antikoerper
Substanzklasse
Viral Protein
Hintergrund
SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 reportedly contains two SH2 domains, both of which are N-terminal to the PTP catalytic domain. SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580. Phosphorylation of Y542 creates an interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an interaction at the C-terminal that stimulates PTP activity. The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2). The homologous phosphorylation site in the PTP2Ci splice variant (isoform 1) is Y546. The specificity of this antibody was validated by confirming, using western blot analysis, that RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542). Synonyms: PTN11, PTP1D, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHP-2, SHPTP2