CAMP Responsive Element Binding Protein 1 (CREB1) (N-Term) Antikörper

Antigen: CAMP Responsive Element Binding Protein 1 (CREB1)

Synonyme: CREB, MGC9284, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, JAL, hJAL, ATCE1, CREB3, CREB4, AIBZIP, CREB1, creb1, MGC52669, creb, MGC69285, MGC55598, zgc:55598, Creb1, DKFZp468J2311

Epitope: N-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Enzyme Immunoassay (EIA), Elektromobilitäts-Experimente (EMSA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN116948

Produktbeschreibung

Produktmerkmale: Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsiveelement-binding protein 1

Weitere Bezeichnung: CREB1 (N-term)

Swiss-Prot: P16220

Immunogen: CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human proteinconjugated to Keyhole Limpet Hemocyanin (KLH).

Format: Liquid

Beschreibung: Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zippertranscription factor that binds the cyclic AMP response element (CRE) and activatestranscription in response to a variety of extracellular signals including neurotransmitters,hormones, membrane depolarization, and growth and neurotrophic factors. Activation ofCREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs viap44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREBwill bind DNA independent of its phosphorylation state, only the phosphorylated form iscompetent as a transcription factor. CREB binding protein (CBP), a transcriptionalcoactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out miceshow diminished learning ability.

Spezifität: This product was prepared from monospecific antiserum by delipidation andImmunoadsorption against an E. coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is

Anwendungen

Applikationshinweise: Western Blot: This antibody was assayed against a lysate of 1 x 10e6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 µl per assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 2.56 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02M Potassium Phosphate, 0.15 Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservative.

Lagerung: Store the antibody at -20°C. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: 6 month from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. Detection occurs using a 1:1,000 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.