Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Ly6g Antikörper (Biotin)

Ly6g Reaktivität: Maus FACS Wirt: Ratte Monoclonal RB6-8C5 Biotin
Produktnummer ABIN114270
  • Target Alle Ly6g Produkte
    Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
    Reaktivität
    • 102
    • 13
    • 1
    Maus
    Wirt
    • 81
    • 20
    • 1
    Ratte
    Klonalität
    • 81
    • 19
    • 1
    Monoklonal
    Konjugat
    • 26
    • 9
    • 9
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Ly6g Antikörper ist konjugiert mit Biotin
    Applikation
    • 78
    • 35
    • 30
    • 29
    • 22
    • 17
    • 11
    • 10
    • 9
    • 8
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Kreuzreaktivität (Details)
    Species reactivity (tested):Mouse.
    Immunogen
    Mouse Granulocytes.
    Klon
    RB6-8C5
    Isotyp
    IgG2b
  • Applikationshinweise
    Flow cytometry. Immunohistochemistry on frozen and paraffin embedded tissues. Western blot. Complement-mediated depletion.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of ABIN114273. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) . 9. Incubate tubes at 4°C for 30-60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Mouse Strain: BALB/cCell
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
    Andere Bezeichnung
    Ly6G / GR1 Neutrophil Marker (Ly6g Produkte)
    Synonyme
    Gr-1 antikoerper, Gr1 antikoerper, Ly-6G antikoerper, lymphocyte antigen 6 complex, locus G antikoerper, Ly6g antikoerper
    Hintergrund
    Synonyms: Gr-1 Granulocyte marker
    Gen-ID
    10090
    UniProt
    P35461
Sie sind hier:
Kundenservice