COL3A1 Antikörper (Chain alpha 1)

Produktnummer ABIN112535

Der Maus Monoklonal anti-COL3A1 Antikörper wird verwendet zum Nachweis von COL3A1 in Proben von Human. Er wurde validiert für IF, IHC (p), EIA, IHC (fro) und RIA.

Kurzübersicht für COL3A1 Antikörper (Chain alpha 1) (ABIN112535)

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COL3A1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Klon: 8D1-8C7

Produktdetails anti-COL3A1 Antikörper

Bindungsspezifität: Chain alpha 1

Spezifität: Human Collagen type III 100 % (solid phase RIA at 1/500 dilution). Type III Collagen, MW 95 kDa, can be observed in skin, cartilage, human placenta and vitreous body. Cross Reactions: Human Collagen I, II, IV and V: < 0,1 % Human Fibronectin and Laminin: < 0,1 %. Does not react with Mouse, Rat, Bovine and Pig.

Aufreinigung: Affinity Chromatography.

Immunogen: Purified collagen type III from human placenta

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. RIA (> 1/1000). Immunflourescence assay: 1/80Immunohistochemistry on Paraffin Sections: 1/1000 (60 min at RT or 2-8 °C over night). Immunohistochemistry on Frozen Sections. Pretreatment for IHC: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding isblocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 %peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human skin and placenta.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.1 mL distilled water.

Konzentration: 1.0 mg/mL

Buffer: PBS without preservatives or BSA.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Details zu COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage, vitreous body, and human placenta.Synonyms: COL3A1

Gen-ID: 1281

NCBI Accession: NP_000081

UniProt: P02461

Pathways: Autophagie, Growth Factor Binding