Integrin beta 2 Antikörper (Activation Site)

Produktnummer ABIN111902

Produktdetails anti-Integrin beta 2 Antikörper

Target: Integrin beta 2 (ITGB2)

Bindungsspezifität: Activation Site

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Spezifität: The antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin b2 subunit, b2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Kreuzreaktivität (Details): Species reactivity (tested):Human. Negative with Porcine.

Aufreinigung: Protein A Affinity Chromatography

Immunogen: Peripheral blood mononuclear cells.

Klon: MEM-148

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: The antibody is excellent marker of activated myeloid cells. Immunoprecipitation. Western Blotting (Non Reducing Conditions). Functional Application: The antibody induces homotypic cell aggregation and high-affinityLFA-1 conformation in resting leukocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 15 mM Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Integrin beta 2 Antikörper (ABIN111902)

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18 / ITGB2 (ITGB2 Produkte)

Synonyme: CD18 antikoerper, LAD antikoerper, LCAMB antikoerper, LFA-1 antikoerper, MAC-1 antikoerper, MF17 antikoerper, MFI7 antikoerper, 2E6 antikoerper, AI528527 antikoerper, Cd18 antikoerper, Lfa1 antikoerper, ITGB2 antikoerper, itgb2 antikoerper, itgb2-a antikoerper, itgb2-b antikoerper, integrin subunit beta 2 antikoerper, integrin beta 2 antikoerper, integrin subunit beta 2 S homeolog antikoerper, ITGB2 antikoerper, Itgb2 antikoerper, itgb2.S antikoerper

Hintergrund: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gen-ID: 3689

NCBI Accession: NP_000202

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation