GFAP Antikörper

Produktnummer ABIN111187

Dieser Maus Monoklonal Antikörper detektiert spezifisch GFAP in WB, IF, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus und Rind (Kuh).

Kurzübersicht für GFAP Antikörper (ABIN111187)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: GF12-24

Produktdetails anti-GFAP Antikörper

Spezifität: GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide Reacting: Mr 50 000 glial filament protein GFP. Tumors Specifically Reacting: Astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas. Tested Reactivities on Cultured Cell Lines: U 333 CG/343MG.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Mouse and Rat.

Aufreinigung: Protein A Chromatography

Immunogen: Intermediate filament cytoskeleton from cultured human glioma cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoblotting. Immunofluorescence Microscopy. Immunohistochemistry. Cytological MaterialImmunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. Working Dilutions: 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation Time: 1 h ar RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytesSynonyms: Glial Fibrillary Acidic Protein

Gen-ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136