FAS Ligand (CD95) Antikörper

Antigen: FAS Ligand (CD95)

Synonyme: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, FAS, LOC574159, fasl, zgc:162027

Klonalität: Monoklonal (GM5F4)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Zell-ELISA (cELISA)

Produktnummer: ABIN108724

Produktbeschreibung

Immunogen: GM5F4 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human FASL extracellular domain. The antibody was purified by protein G affinity chromatography from cell culture supernatants and is routin

Format: Purified

Isotyp: IgG1

Klon: GM5F4

Beschreibung: The FAS ligand (FASL, CD95), a member of the tumour necrosisfactor family, induces apoptosis in FAS-bearing cells. FASL is a type II membrane receptor with a soluble form that can be released into the extracellular fluid by proteolytic processing. Northern hybridisation revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several non lymphoid tissues, such as testis.

Spezifität: GM5F4 recognises FASL transiently expressed on the cell surface of transfected BOSC cells (Fig. 1).

Synonyme: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, FAS, LOC574159, fasl, zgc:162027

Anwendungen

Applikationshinweise: GM5F4 has been tested for the use in flow cytometry and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concent

Lagerung: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Forschungsgebiet: Apoptose/Nekrose, Apoptose/Nekrose, Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Specificity testing of GM5F4. BOSC cells were transiently transfected with an expression vector for the extracellular domain of FASL as well as an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody (B, open c