Fodrin Antikörper

Antigen: Fodrin

Klonalität: Monoklonal (AA6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN108428

Produktbeschreibung

Immunogen: Monoclonal antibody to a-fodrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation were used as immunogen.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: AA6

Beschreibung: Monoclonal Antibody to Human Cytoskeletal Polypeptides

Spezifität: The antibody is specific to the 240 kD a-fodrin molecule of all mammalian nonerythroid cells and chicken a-spectrin. The antibody crossreacts with human, rat, chicken, rabbit and guinea pig

Anwendungen

Applikationshinweise: No reactivity with paraffin sections. Working dilution in western blotting is at least 1:1000

Lagerung: This product should be stored at +2 C to +8 C

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Eskelinen S, Huotari V, Sormunen R et al.: "Low intracellular pH induces redistribution of fodrin and instabilization of lateral walls in MDCK cells." in: J Cell Physiol, Vol. 150, Issue 1, pp. 122-33, 1992 (PubMed).

Huotari V, Sormunen R, Lehto VP et al.: "Different organizational states of fodrin in cultured MDCK cells are induced by treatment with low pH, calmodulin antagonist TFP, and tumor promoter PMA." in: J Cell Physiol, Vol. 153, Issue 2, pp. 340-52, 1992 (PubMed).

Ylikoski J, Pirvola U, Narvanen O et al.: "Nonerythroid spectrin (fodrin) is a prominent component of the cochlear hair cells." in: Hear Res, Vol. 43, Issue 2-3, pp. 199-203, 1990 (PubMed).

Reima I, Lehtonen E, Virtanen I et al.: "The cytoskeleton and associated proteins during cleavage, compaction and blastocyst differentiation in the pig." in: Differentiation, Vol. 54, Issue 1, pp. 35-45, 1993 (PubMed).

Sormunen R, Paakko P, Palovuori R et al.: "Fodrin and actin in the normal, metaplastic, and dysplastic respiratory epithelium and in lung carcinoma." in: Am J Respir Cell Mol Biol, Vol. 11, Issue 1, pp. 75-84, 1994 (PubMed).

Sormunen R: "Alpha-spectrin in detergent-extracted whole-mount cytoskeletons of chicken embryo heart fibroblasts." in: Histochem J, Vol. 25, Issue 9, pp. 678-86, 1993 (PubMed).

Vaaraniemi J, Huotari V, Lehto VP et al.: "The effects of PMA and TFP and alterations in intracellular pH and calcium concentration on the membrane associations of phospholipid-binding proteins fodrin, protein kinase C and annexin II in cultured MDCK cells." in: Biochim Biophys Acta, Vol. 1189, Issue 1, pp. 21-30, 1994 (PubMed).

Sormunen R, Eskelinen S, Lehto VP: "Bile canaliculus formation in cultured HEPG2 cells." in: Lab Invest, Vol. 68, Issue 6, pp. 652-62, 1993 (PubMed).

Huotari V, Vaaraniemi J, Lehto VP et al.: "Regulation of the disassembly/assembly of the membrane skeleton in Madin-Darby canine kidney cells." in: J Cell Physiol, Vol. 167, Issue 1, pp. 121-30, 1996 (PubMed).

Tuominen H, Sormunen R, Kallioinen M: "Non-erythroid spectrin (fodrin) in cutaneous tumours: diminished in cell membranes, increased in the cytoplasm." in: Br J Dermatol, Vol. 135, Issue 4, pp. 576-80, 1996 (PubMed).

Vaaraniemi J, Huotari V, Lehto VP et al.: "Effect of PMA on the integrity of the membrane skeleton and morphology of epithelial MDCK cells is dependent on the activity of amiloride-sensitive ion transporters and membrane potential." in: Eur J Cell Biol, Vol. 74, Issue 3, pp. 262-72, 1997 (PubMed).

Sormunen RT, Leong AS, Vaaraniemi JP et al.: "Immunolocalization of the fodrin, E-cadherin, and beta-catenin adhesion complex in infiltrating ductal carcinoma of the breast-comparison with an in vitro model." in: J Pathol, Vol. 187, Issue 4, pp. 416-23, 1999 (PubMed).