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ZNF808 Protein (AA 1-903) (Strep Tag)

Recombinant ZNF808-Protein exprimiert in Cell-free protein synthesis (CFPS).
Produktnummer ABIN3075989
ab
15.754,29 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt
250 μg
Lieferung nach: Deutschland
Lieferung in 51 bis 61 Werktagen

Kurzübersicht für ZNF808 Protein (AA 1-903) (Strep Tag) (ABIN3075989)

Target

ZNF808 (Zinc Finger Protein 808 (ZNF808))

Protein-Typ

Recombinant

Spezies

Human

Quelle

Cell-free protein synthesis (CFPS)

Applikation

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse

custom-made
  • Proteineigenschaft

    AA 1-903

    Aufreinigungstag / Konjugat

    Dieses ZNF808 Protein ist gelabelt mit Strep Tag.

    Sequenz

    MLREEAAQKR KGKESGMALP QGRLTFRDVA IEFSLAEWKF LNPAQRALYR EVMLENYRNL EAVDISSKHM MKEVLSTGQG NREVIHTGTL QRHQSYHIGD FCFQEIEKEI HNIEFQCQED ERNGHEAPTT KIKKLTGSTD QHDHRHAGNK PIKDQLGSSF YSHLPELHIF QIKGEIANQL EKSTSDASSV STSQRISCRP QIHISNNYGN NPLNSSLLPQ KQEVHMREKS FPCNESGKAF NCSSLLRKHQ IPHLGDKQYK CDVCGKLFNH KQYLACHRRC HTGEKPYKCK ECGKSFSYKS SLTCHHRLHT GVKPYKCNEC GKVFRQNSAL VIHKAIHTGE KPYKCNECGK AFNQQSHLSR HQRLHTGVKP YKCKICEKAF ACHSYLANHT RIHSGEKTYK CNECGKAFNH QSSLARHHIL HTGEKPYKCE ECDKVFSQKS TLERHKRIHT GEKPYKCKVC DTAFTCNSQL ARHRRIHTGE KTYKCNECRK TFSRRSSLLC HRRLHSGEKP YKCNQCGNTF RHRASLVYHR RLHTLEKSYK CTVCNKVFMR NSVLAVHTRI HTAKKPYKCN ECGKAFNQQS HLSRHRRLHT GEKPYKCEAC DKVFGQKSAL ESHKRIHTGE KPYRCQVCDT AFTWNSQLAR HTRIHTGEKT YKCNECGKTF SYKSSLVWHR RLHGGEKSYK CKVCDKAFVC RSYVAKHTRI HSGMKPYKCN ECSKTFSNRS SLVCHRRIHS GEKPYKCSEC SKTFSQKATL LCHRRLHSGE KPYKCNDCGN TFRHWSSLVY HRRLHTGEKS YKCTVCDKAF VRNSYLARHI RIHTAEKPYK CNECGKAFNE QSHLSRHHRI HTGEKPYKCE ACDKVFSRKS HLKRHRIIHT GEKPYKCNEC GKAFSDRSTL IHHQAIHGIG KFD
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Produktmerkmale

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Applikationshinweise

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C.

    Haltbarkeit

    12 months
  • Target

    ZNF808 (Zinc Finger Protein 808 (ZNF808))

    Andere Bezeichnung

    ZNF808

    Hintergrund

    Zinc finger protein 808,FUNCTION: Transcriptional repressor that targets mainly transposable elements (PubMed:37973953). Primarily targets the long terminal repeat of endogenous retroviruses classified as MER11 elements which comprise subfamilies A, B and C (PubMed:37973953). May silence transposable elements through the establishment of heterochromatin-associated trimethylation of 'Lys-9' of histone H3 (H3K9me3) (PubMed:37973953). Can also bind to certain gene promoters and other genomic regions (PubMed:37973953). Represses transcription of specific MER11 elements during differentiation toward pancreatic lineages in early pancreas development (PubMed:37973953). By repressing transcription, prevents a liver gene expression program from being aberrantly activated during pancreas differentiation (PubMed:37973953). {ECO:0000269|PubMed:37973953}.

    Molekulargewicht

    104.8 kDa

    UniProt

    Q8N4W9
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