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- Wirt
- Ratte
- Applikation
- Western Blotting (WB)
- Produktmerkmale
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The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: rat rat adrenal, 3: cerebellum, 4: cerebrum, 5: small intestine, 6: skeletal muscle, 7: stomach, 8: testis, and 9: thymus
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Hamster Complement (Lyophilized) With Diluent
Wirt: Hamster Normal Complement
Guinea Pig Liver Acetone Tissue PowderIA Wirt: Meerschweinchen Normal Liver
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- Applikationshinweise
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The blot can be reused after stripping.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 4 °C.
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