Kurzübersicht für Ratte Normal Tissue Blot II (ABIN2455975)
Wirt
Applikation
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Produktmerkmale
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The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: rat rat adrenal, 3: cerebellum, 4: cerebrum, 5: small intestine, 6: skeletal muscle, 7: stomach, 8: testis, and 9: thymus
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Rat Tail
Wirt: Ratte
Sheep Red Blood Cell (RBC) 10% Washed Pooled CellsCA, FACS Wirt: Schaf Blood
Guinea Pig Spinal CordWirt: Meerschweinchen Normal Spinal Cord
Hamster Complement (Lyophilized) with DiluentWirt: Hamster Complement
Guinea Pig Complement (Lyophilized) with DiluentELISA Wirt: Meerschweinchen Complement
Guinea Pig Complement (Fresh Frozen)CA, ELISA, FACS Wirt: Meerschweinchen Complement
Rabbit Blood in Sodium CitrateWirt: Kaninchen Blood
Chicken Red Blood Cells (RBC) 10% Washed Pooled CellsChIP Wirt: Huhn Blood
Human Red Blood Cells (RBC) 10% Washed Pooled CellsWirt: Human Blood
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Applikationshinweise
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- The blot can be reused after stripping.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4 °C.
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