Human Normal Tissue Blot VI
Kurzübersicht für Human Normal Tissue Blot VI (ABIN2455967)
Wirt
Applikation
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Produktmerkmale
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: left cerebellum, 3: right cerebellum, 4: frontal lobe, 5: occipital lobe, 6: parietal lobe, 7: whole eye, 8: spinal cord, 9: temporal lobe, and 10: thalamus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Sheep Red Blood Cell (RBC) 10% Washed Pooled Cells
CA, FACS Wirt: Schaf Blood
Guinea Pig Spinal CordWirt: Meerschweinchen Normal Spinal Cord
Hamster Complement (Lyophilized) with DiluentWirt: Hamster Complement
Guinea Pig Complement (Lyophilized) with DiluentELISA Wirt: Meerschweinchen Complement
Guinea Pig Complement (Fresh Frozen)CA, ELISA, FACS Wirt: Meerschweinchen Complement
Rabbit Blood in Sodium CitrateH Wirt: Kaninchen Blood
Chicken Red Blood Cells (RBC) 10% Washed Pooled CellsChIP Wirt: Huhn Blood
Human Red Blood Cells (RBC) 10% Washed Pooled CellsWirt: Human Blood
Sheep Red Blood Cell (RBC) 100% Washed Pooled CellsFunc, IF Wirt: Schaf Blood
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Applikationshinweise
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- The blot can be reused after stripping.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4 °C.
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