Human Lung Tumor Blot V
WB
Wirt: Human
Produktnummer ABIN2455961
792,39 €
Zzgl. Versandkosten 20,00 € und MwSt
1 applications
Lieferung nach:
Deutschland
Lieferung in 6 bis 9 Werktagen
Kurzübersicht für Human Lung Tumor Blot V (ABIN2455961)
Wirt
Human
Applikation
Western Blotting (WB)
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Produktmerkmale
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 differentiated carcinoma, and 1 pleomorphic carcinoma.
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: A549, 3: small cell carcinoma, 4: matched normal lung tissue, 5: differentiated carcinoma, 6: matched normal lung tissue, 7: pleomorphic carcinoma, and 8: matched normal lung tissue.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Applikationshinweise
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- The blot can be reused after stripping.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4 °C.
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