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Human OCT4 Protein expressed in HEK-293 Cells - ABIN2727872
Soufi, Garcia, Jaroszewicz, Osman, Pellegrini, Zaret: Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming. in Cell 2015
OCT4B(19kDa) may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.
NKX3-1 substitutes for exogenous OCT4 to reprogram both mouse and human fibroblasts at comparable efficiencies and generate fully pluripotent stem cells.
These results suggest that the Oct4 gene may regulate Neuroblastic tumours (NBT) pathogenic differentiation pathways, and should thus be considered as a target for knockdown when developing novel therapies for high-risk NBT patients.
The PAX5-OCT4-PRDM1 proteins form a core transcriptional network that activates germline and represses somatic programmes during human germ cell differentiation.
Nitric oxide (NO) promotes the cancer stem cells -regulatory activity of Oct4 through a mechanism that involves complex formation between Oct4 and the scaffolding protein caveolin-1 (Cav-1). In the absence of NO, Oct4 forms a molecular complex with Cav-1, which promotes the ubiquitin-mediated proteasomal degradation of Oct4.
Correlation analysis revealed that OCT4B expression was significantly associated with the SLUG level in lung tumors. These results provide novel insights into OCT4B-mediated oncogenesis in cancer dissemination.
The authors demonstrate that expression of CD90 and OCT4 are increased in hepatocellular carcinoma and higher expression of these two proteins is correlated with poor prognosis of HCC patients.
Results suggest that DNA hypomethylation may be a key mechanism underlying the up-regulation of OCT4 in the recurrence of glioma, which facilitates the understanding of the role of stem cells.
We have therefore modeled the structure of the complex of the whole Oct4 POU domain and importin alpha2 using protein-protein docking and molecular dynamics. The model explains how the Ebola virus VP24 protein has a negative effect on the nuclear import of STAT1 by importin alpha but not on Oct4, and how Nup 50 facilitates cargo release from importin alpha
Oct4 was related to tumor differentiation and later Dukes stage in colon cancer.Oct4 expression was a potential prognostic indicator for worse prognosis in right-sided colon cancer, but not in left-sided colon cancer.
Study established a relationship between OCT4 and SMAD3 heterodimers formation and Snail, Slug, and CXCL13 transcription promotion mediating breast cancer progression.
Metastatic double primary endometrioid endometrial and ovarian carcinoma sections expressed a higher level of OCT4 compared to the corresponding DPEEOC tissues.
Cripto-1 expression is increased by OCT4 overexpression, but decreased by shRNA-mediated OCT4 knockdown. OCT4 overexpression significantly activates Cripto-1 transcriptional activity. A 5'-upstream minimal promoter sequence in the gene-encoding Cripto-1 is significantly activated by OCT4 overexpression.
Multivariate Cox's proportional hazards regression analyses revealed that OCT4 mRNA high expression was an independent predictive factor for shorter EFS and OS in AML patients. Conclusion OCT4 correlates with presence of CK, FLT3-ITD mutation and poorer risk stratification, and it could be served as a convincing biomarker for predicting unfavourable prognosis in AML patients.
GCNF acts as a transcriptional repressor in the regulation of OCT4 gene expression through cooperative interaction with three NR binding elements in pluripotent NCCIT cells.
Study shows that irrespective of tumor type or clinicopathological status, breast cancer cells expressed high levels of Oct3/4 at mRNA and protein levels.
Tumor tissue OCT4 expression was positively correlated with histologic grade, pathological tumor size and prognosis in surgical patients with triple-negative breast cancer.
Radiation resistance tumors have upregulated Onzin and POU5F1 expression.
verification of the presence of stem cell-like cells in the epithelial component through the immunopositivity to Oct-4 and CD44 in benign odontogenic lesions of variable biological behaviors
Oct4 plays a vital role in the malignant progression of HCC cells through the survivin/STAT3 signaling pathway.
Cytoplasmic CD44 and absence of nuclear Oct3/4 suggest that the cells of cardiac sarcomas may represent 'daughter' stem cells that no longer have the capacity for tumour initiation, but have subsequently developed new lines of partial differentiation.
Lack of restricted OCT4 protein, and inner cell mass localization of NANOG in primate blastocysts, suggests that NANOG may determine inner cell mass fate more specifically during primate development.
Western blot analysis were conducted on developing hatchlings with Oct4 antibody and at 13.6 and 21.6mugL(-1)dose,it showed over expression elucidating stimulatory role of nanosilver. In silico analysis depicted a firm interaction of nanosilver with Oct4 revealing their key role in growth stimulation of developing embryos.
The authors show, at single-cell resolution in vivo, that Pou5f3 complexes with Nanog to pattern mesendoderm differentiation at the blastula stage.
Data suggest that, in developing gastrula, Znfl1 controls developmental gene expression of Hoxb1b in embryonic posterior neuroectoderm by acting upstream of Pou5f3 and Sall4; these proteins appear to be involved in neurogenesis. (Znfl1 = zinc finger-like gene 1; Hoxb1b = homeobox B1b protein; Pou5f3 = POU domain class 5 transcription factor 3; Sall4 = spalt-like transcription factor 4)
Regulation of mych by Pou5f1 appears to be direct transcriptional activation.
The posttranslational modification by phosphorylation opens the possibility that Pou5f1 may be subject to temporal or region specific modulation of its activity or stability by embryonic signaling mechanisms.
discuss mechanistic implications of simultaneous activation of transcriptional targets by ubiquitous, like Pou5f1, and region-specific inducers, emerging as a common regulatory motif in early development
maternal Nanog, Pou5f1 and SoxB1 are required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression
thses data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control.
The defects due to HEP induction were rescued by introducing wild-type pou2 mRNA before the heat treatments.
Vox plays a key role downstream of BMP signals in regulating the capacity of Nodal to induce endoderm versus mesoderm by modulating the activity of the Casanova/Pou2 regulatory system.
Pou2 functions in multiple aspects of vertebrate development, especially in the binary decision of the mesendoderm to mesoderm and endoderm in different ways depending on the developmental stage.
show that Pou5f1 binds to phylogenetically conserved Oct/Pou5f1 sites in the vox promoter, both in vivo and in vitro
The temporospatial structure of the zebrafish Pou5f1 target networks may explain aspects of the evolution of the mammalian stem cell networks.
Pou2 is essential for endoderm formation. Embryos devoid of maternal and zygotic pou2 lack endodermal precursors.
Data show that embryos genetically depleted of both maternal and zygotic pou2 function exhibit extreme dorsoventral patterning defects and, independently, a blastoderm-specific arrest of epiboly.
Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defects of Pou5f1/Oct4 deficient MZspg zebrafish embryos.
These data provide a scheme wherein pou2/pou5f1 expression in zebrafish embryos is regulated by both an autoregulatory loop and repression by RA emanating from the posterior mesoderm.
ubiquitous expression of the protein in early (Day 4) in vivo-developed rabbit blastocysts and its restriction to the embryonic disk by Day 6
sequences of mRNA and translated protein of the newly identified genes and those of POU5F1 were aligned to their mammalian orthologs to determine the degree of evolutionary conservation
Higher values of OCT-4 expression were observed in embryos and endometrial tissue in females reproduced under heat conditions.
protein expression during rabbit embryonic development: investigation of temporal and spatial relationship of expression of Oct-4, Cdx-2 (caudal type homeobox transcription factor 2) and histone H4 (acetylated at lysine 5) in morula/blastocysts
The signal may have reflected the regulation of Oct-4 through enhancer switching and therefore may be related to cell lineage formation in rabbit embryos.
Cloning of POUF5F1 cDNA and its genomic organization, chromosome localization and expression in adult tissues and embryonic stem cells has been reported.
The POU5F1 gene is strictly regulated during early embryo development.
Knockout of Oct4 in perivascular cells significantly impairs perivascular cell migration, increases perivascular cell death, delays endothelial cell migration, and promotes vascular leakage following corneal angiogenic stimulus. Knockout of Oct4 in perivascular cells also impairs perfusion recovery and decreases angiogenesis following hindlimb ischemia.
The effect of p53 on the capacity of cardiogenic transdifferentiation is evaluated using p53 wild-type (p53(+/+)), p53 heterozygous mutant (p53(+/-)), and p53 homozygous mutant (p53(-/-)) embryonic fibroblasts (MEFs). The effect of an important reprogramming factor Oct4 on cardiac transdifferentiation was also evaluated in the allelic series of p53 MEFs.
we demonstrated that hypoxia significantly induced the GSC mesenchymal transition, increased the expression levels of the pluripotent transcription factor OCT4 and migration-associated proteins..he underlying mechanism involved significant IGF-1/IGF-1R activation of OCT4/CXCR4 expression through HIF-2alpha regulation.
Oct4 is involved in DNA methylation through the regulation of Dnmt1 transcription, especially during the early stages of mouse pre-implantation embryo development.
Zfp127 is a novel regulator of Oct4, and may become a potent target to improve cellular reprogramming.
Here we describe an optimized DamID method coupled with next-generation sequencing (DamID-seq) in mouse cells and demonstrate the identification of the binding sites of two TFs, POU5F1 (also known as OCT4) and SOX2, in as few as 1000 embryonic stem cells (ESCs) and neural stem cells (NSCs), respectively.
Data show that OCT4 protein impedes mouse embryonic stem cells (mESCs) differentiation despite MITF transcription factor (MITF) expression.
These results indicate a role for Oct4 in orchestrating multiple fates and enabling expansion, correct patterning and lineage choice in the postimplantation epiblast.
Acidic pH induced OCT-4 expression in fibroblasts and stromal cells in tumor microenvironment.
Threonine(343) based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency and interaction with SOX2.
These results demonstrated that OCT4 phosphorylation on serine 347 by JNKs plays an important role in its stability, transcriptional activities, and self-renewal of mouse ESCs
the epigenetic status of Oct4 and Nanog genes confirm that pre-induced pluripotent stem cells belong to an RVTg+OCT4+NANOG- state
These observations support a role for YY1 meditating and/or regulating the interaction of OCT4 and SOX2 at a posttranscriptional level.
The results revealed that the synthesized complex decoy can concomitantly target Sox2 and Oct4, which subsequently represses the stemness properties of mESCs compared to controls through decreasing cell viability, arresting cell cycle in G0 /G1 phases, inducing apoptosis, and modulating differentiation in mESCs despite the presence of 2i/LIF in cell culture.
Tip110 deletion impaired embryonic and stem cell development involving downregulation of stem cell factors Nanog, Oct4, and Sox2.
Spermatogonial stem cells and progenitors are refractory to reprogramming to pluripotency by the transcription factors Oct3/4, c-Myc, Sox2 and Klf4.
Nanog, Oct4 and Tet1 interplay in establishing stem cell pluripotency has been described.
The positive feedback regulation of OCT4 and c-JUN, resulting in the continuous expression of oncogenes such as c-JUN, seems to play a critical role in the determination of the cell fate decision from induced pluripotent stem cells to cancer stem cells in liver cancer.
Activin A and overexpression of SMAD2/3 significantly promoted expressions of porcine NANOG and OCT4,maintaining induced pluripotent stem cell self-renewal through up-regulation of Nanog/OCT4 expression.
Our results indicate that continuous expression of OCT-4 in blastomeres is essential for trophectoderm formation of porcine embryos.
These findings suggest that the 12-bp indel polymorphism of the Oct4 gene might be a potential DNA marker for selecting preferred individuals in relation to reproductive traits in pig marker-assisted selection breeding.
Oct4-overexpression enhances porcine ovarian stem cell differentiation in to oocyte-like cells.
showed novel molecular regulation of CDX2 on Oct4, and provided important clues for clarifying the mechanism of interaction between CDX2 and Oct4 in embryo of mammals other than mouse
Overexpression of Sox2 or Oct4 in bone mesenchymal stem cells in culture media containing a basic fibroblast growth factor results in higher proliferation and differentiation compared to controls.
Oct4 positive stem/progenitor swine lung epithelial cells are targets for influenza virus replication.
study shows that localization of octamer-binding protein 4(OCT4) is associated with an embryonic stem cell (ESC)-like morphology from porcine inner cell mass
OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo.
cells isolated from umbilical cord express three transcription factors,Oct-4, Sox-2 & Nanog, found in pluripotent stem cell markers both at the mRNA and protein level.
in the forming endoderm at the primitive streak stage, OCT4 was detectable in potential primordial germ cells only; in the ectoderm and mesodermal cell lineages OCT4 was cleared at the neural groove and somite stage, respectively
In OCT4 KO morulae (day 5), approximately 70% of the nuclei were OCT4 positive, indicating that maternal OCT4 mRNA partially maintains OCT4 protein expression during early development.
The use of a clonal cell populations with low senescence level, normal karyotype, and highest POU5F1 expression levels did not improve embryo development rates or quality following SCNT. As previously suggested, this study supports the notion that levels of POU5F1 expression in the donor nucleus do not impact the Somatic cell nuclear transfer results.
we concluded that OCT4 expression in somatic cells is not a good prognosis marker for selecting cell lines.
analysis of pluripotency gene expression of OCT4, SOX2 and NANOG and mRNA levels of some of their downstream targets in bovine oocytes and early embryos
Oct4 exhibited significant hypermethylation in sperm compared with that in oocytes
In contrast to protein distribution, regulation of Oct4 transcription is conserved between mammalian species.
analysis of CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses
The restoration of pluripotency can be directly observed in living cells or SCNT embryos from such Pou5f1-EGFP transgenic fetuses.
The expression of glycolysis-related genes about PGAM1, KPYM2 and HXK2 in CiPSC-like colonies formation groups was significantly higher than their parental fibroblasts, but not in the non-CiPSC-like colonies formation group.
Oct4 cDNA and a 2.8-kb regulatory region upstream of its start codon were isolated and characterized
evaluated pattern of POU5F1 expression during early embryo development;study shows POU5F1 protein is present in cytoplasm and nucleus of immature oocytes, decreases during embryo development to day 5, then increases again within embryonic nuclei [POU5F1]
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6\;22)(p21\;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
POU domain transcription factor OCT4
, POU domain, class 5, transcription factor 1
, POU-type homeodomain-containing DNA-binding protein
, octamer-binding protein 3
, octamer-binding protein 4
, octamer-binding transcription factor 3
, octamer-binding transcription factor-3
, POU class 5 homeobox 1
, POU domain class 5 transcription factor 1
, POU domain gene 2
, spiel ohne grenzen
, spiel ohne grenzen/pou2
, octamer binding transcription factor 4
, Octamer-binding transcription factor 3
, octamer-binding transcription factor 4