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Slc6a20b Protein (AA 1-635) (Strep Tag)

Dieses Recombinant Slc6a20b-Protein wird in Cell-free protein synthesis (CFPS) produziert.
Produktnummer ABIN3132156
ab
15.754,29 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt
250 μg
Lieferung nach: Deutschland
Lieferung in 51 bis 61 Werktagen

Kurzübersicht für Slc6a20b Protein (AA 1-635) (Strep Tag) (ABIN3132156)

Target

Slc6a20b (SLC6A20B) (Solute Carrier Family 6 (Neurotransmitter Transporter), Member 20B (SLC6A20B))

Protein-Typ

Recombinant

Spezies

Maus

Quelle

Cell-free protein synthesis (CFPS)

Applikation

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse

custom-made
  • Proteineigenschaft

    AA 1-635

    Aufreinigungstag / Konjugat

    Dieses Slc6a20b Protein ist gelabelt mit Strep Tag.

    Sequenz

    MESPSAHAVS LPEDEELQPW GGAGGPGQHP GRPRSTECAH PGVVEKVRPK WDNPLQFLLV CISYAVGLGN VWRFPYLCQM YGGGNFLVPY IIMLIVEGMP LLYLELAVGQ RMRQGSIGAW RTISPYLSGV GIASLVVSFL ASVYFNVINT WALWYLFHSF QDPLPWSVCP LNSNHTGYDE ECEKASSTQY FWYRKTLNIS PSIQENGGVQ WEPALCLTLA WLMVYLCILR GTESTGKVVY FTTSLPYFVL IIYLVRGLTL HGATNGLAYM FTPKIEQLAN PKAWINAATQ IFFSLGLGCG GLIAFASYNE PSNDCQKHAL IVSVINSTTA IFSSIVTFSI YGFKATFNYE NCLNKVILLL TNSFDLEDGF LTVSNLEEVK NYLASTYPNK YSEVFPHIRN CSLESELDTA VQGTGLAFIV YTEAIKNMEV SQLWSVLYFF MLLTLGMGSM VGTGTAILTP LTDSKIISSY LPKEAISGLV CLLNCAIGMV FTMEAGNYWF DLFNDYTATL SLLLIVLVET IAVCYVYGLK RFESDLRAMT GRTLSWYWKV MWAFVSPLLI VGLFIFYLSD YILTGTLQYQ AWDATQGHVV TKDYPTYALA VIGLLVASST MCIPLVALGT FVTRHFKIRE QFSAA
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Produktmerkmale

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Applikationshinweise

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C.

    Haltbarkeit

    12 months
  • Target

    Slc6a20b (SLC6A20B) (Solute Carrier Family 6 (Neurotransmitter Transporter), Member 20B (SLC6A20B))

    Andere Bezeichnung

    Slc6a20b

    Hintergrund

    Sodium- and chloride-dependent transporter XTRP3B (IMINO-K) (Solute carrier family 6 member 20B),FUNCTION: Does not show transporter activity with a range of tested amino acids including proline, glutamine, glutamic acid, leucine, alanine, histidine, glycine and arginine. {ECO:0000269|PubMed:15689184}.

    Molekulargewicht

    70.7 kDa

    UniProt

    O88575
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