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Normal Ratte Serum

BI, ELISA, ICC, IF, IHC Wirt: Ratte
Produktnummer ABIN7232258

Kurzübersicht für Normal Ratte Serum (ABIN7232258)

Target

Normal Serum

Wirt

Ratte

Applikation

BioImaging (BI), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
  • Verwendungszweck

    Normal Rat Serum

    Aufreinigung

    Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2

    Reinheit

    Whole serum
  • Applikationshinweise

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).

    Kommentare

    Blocking Serum

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    10 mM sodium phosphate, 0.15 M sodium chloride with 0.05 % sodium azide as preservative, pH 7.2

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
  • Target

    Normal Serum

    Hintergrund

    High quality Normal rat serum processed from non-immunized normal adult rats is used as a blocking agent or as a negative control for immunoassays.,Normal rat serums, which are from non-immuned rat hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5 % -20 % (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.,Serum
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