Ziege anti-Human Ig (Chain kappa), (Light Chain) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN969902

Kurzübersicht für Ziege anti-Human Ig (Chain kappa), (Light Chain) Antikörper (Alkaline Phosphatase (AP)) (ABIN969902)

Target: Ig

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Bindungsspezifität: Chain kappa, Light Chain

Reaktivität: Human

Wirt: Ziege

Spezifität: Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

Kreuzreaktivität (Details): Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins

Produktmerkmale: Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase

Aufreinigung: Affinity purified using solid phase human Kappa (κ) Chain

Reinheit: > 95 % based on SDS-PAGE

Immunogen: Purified human kappa (kappa) light chain

Anwendungsinformationen

Applikationshinweise: This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Kommentare:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.50 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Prepare working dilution prior to use and then discard.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulin Ig