Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN965309

Hersteller Produkt- Nr.: 803-4302

Kurzübersicht für Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN965309)

Target: IgG

Fragment: Fab fragment

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Huhn

Wirt: Kaninchen

Hersteller Produkt- Nr.: 803-4302

Hersteller: Rockland

Verwendungszweck: Fab Chicken IgG (H&L) Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.  No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).

Produktmerkmale: Fab Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Immunogen: Optional[Immunogen]: Chicken IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:4,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Loch, Strickler: "A microarray of ubiquitylated proteins for profiling deubiquitylase activity reveals the critical roles of both chain and substrate." in: Biochimica et biophysica acta, Vol. 1823, Issue 11, pp. 2069-78, (2012) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Fab Anti-Chicken IgG Peroxidase Antibody generated in rabbit detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.