Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper (PE)

Produktnummer ABIN965244

Hersteller Produkt- Nr.: 712-108-002

Kurzübersicht für Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper (PE) (ABIN965244)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: PE

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ratte

Wirt: Ziege

Hersteller Produkt- Nr.: 712-108-002

Hersteller: Rockland

Verwendungszweck: F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Produktmerkmale: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.

Immunogen: Optional[Immunogen]: Rat IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Antibody has been tested by western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW CYTOMETRY 1:100 - 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Rat IgG (H&L) Antibody generated in goat detects immunoglobulin g from Rat, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Phycoerythrin.