Ziege anti-Maus IgM Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN965173

Hersteller Produkt- Nr.: 710-1507

Kurzübersicht für Ziege anti-Maus IgM Antikörper (Alkaline Phosphatase (AP)) (ABIN965173)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 710-1507

Hersteller: Rockland

Verwendungszweck: F(ab')2 Mouse IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgM and Mouse Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other mouse heavy or light chain proteins.

Produktmerkmale: F(ab')2 Anti-Mouse IgG (H&L) Alkaline Phosphatase Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: F(ab')2 Anti-Mouse IgM (mu chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgM whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Mouse IgM (mu chain) Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 μg of Mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Mouse IgM (mu chain) Antibody. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.