Ziege anti-Maus IgM Antikörper

Produktnummer ABIN965158

Hersteller Produkt- Nr.: 710-1107

Kurzübersicht für Ziege anti-Maus IgM Antikörper (ABIN965158)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 710-1107

Hersteller: Rockland

Verwendungszweck: F(ab')2 Mouse IgM (mu chain) Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Produktmerkmale: F(ab')2 Mouse IgG (H&L) Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: F(ab')2 anti-Mouse IgM antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Mouse IgM was produced by repeated immunization with Mouse IgM heavy chain in goat.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 anti-Mouse IgM antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:200 - 1:2,000 ELISA Dilution: 1:2,000 - 1:8,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Mouse IgM antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.