Ziege anti-Human IgG (F(ab')2 Region) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN965118

Hersteller Produkt- Nr.: 709-1618

Kurzübersicht für Ziege anti-Human IgG (F(ab')2 Region) Antikörper (Biotin) - Preadsorbed (ABIN965118)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 709-1618

Hersteller: Rockland

Verwendungszweck: F(ab')2 Human IgG F(ab')2 Antibody Biotin Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Hs Ms & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse, and Rat Serum Proteins.

Produktmerkmale: F(ab')2 Anti-Human IgG F(c) Biotin Antibody generated in goat detects Human F(c). Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human IgG F(ab')2 fragment

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Human IgG F(ab')2 Biotin Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:40,000 ELISA Dilution: 1:500,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Human IgG F(ab')2 Biotin Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.