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Ziege anti-Human IgG (Heavy & Light Chain) Antikörper - Preadsorbed

ELISA, IHC, WB, DB Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
Produktnummer ABIN965091
Hersteller Produkt- Nr.: 709-1116
  • Target Alle IgG Produkte
    IgG
    Fragment
    F(ab')2 fragment
    Bindungsspezifität
    • 2397
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 729
    • 696
    • 598
    • 468
    • 399
    • 240
    • 208
    • 175
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 1775
    • 1547
    • 569
    • 261
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Ziege
    Klonalität
    • 3406
    • 182
    • 16
    • 1
    Polyklonal
    Applikation
    • 2061
    • 1987
    • 1865
    • 1806
    • 998
    • 920
    • 896
    • 487
    • 423
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 58
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Hersteller Produkt- Nr.
    709-1116
    Hersteller
    Rockland
    Verwendungszweck
    F(ab')2 Human IgG Antibody Pre-Adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against Bv Hs Ms & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins.
    Produktmerkmale
    F(ab')2 Anti-Human IgG (gamma chain) Antibody generated in goat detects human immunoglobulin g (gamma chain). Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    Aufreinigung
    F(ab')2 Anti-Human IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG whole molecule in goat.

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise
    Application Note: F(ab')2 Anti-Human IgG (H&L) Antibody has been tested by ELISA and dot blot and is suitable for ELISA, immunoblotting, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:42,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    F(ab')2 Anti-Human IgG (H&L) Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 Anti-Human IgG (H&L) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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