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Kaninchen anti-Syrischer Goldhamster IgG (Heavy & Light Chain) Antikörper

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
Produktnummer ABIN965065
Hersteller Produkt- Nr.: 707-401-002

Kurzübersicht für Kaninchen anti-Syrischer Goldhamster IgG (Heavy & Light Chain) Antikörper (ABIN965065)

Target

IgG

Fragment

F(ab')2 fragment

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Applikation

  • 2119
  • 2052
  • 1900
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp

IgG
  • Bindungsspezifität

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reaktivität

    • 752
    • 706
    • 609
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Syrischer Goldhamster

    Wirt

    • 1787
    • 1582
    • 569
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Kaninchen

    Hersteller Produkt- Nr.

    707-401-002

    Hersteller

    Rockland

    Verwendungszweck

    F(ab')2 Golden Syrian Hamster IgG (H&L) Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c).  Limited reactivity will occur with Armenian Hamster IgG.

    Produktmerkmale

    F(ab')2 Anti-Golden Syrian Hamster IgG Rhodamine Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

    Aufreinigung

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    Sterilität

    Sterile filtered

    Immunogen

    Optional[Immunogen]: Golden Syrian Hamster IgG whole molecule
  • Applikationshinweise

    Application Note: F(ab')2 Anti-Golden Syrian Hamster IgG Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    Haltbarkeit

    12 months
  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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