Ziege anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper (Biotin)

Produktnummer ABIN965059

Hersteller Produkt- Nr.: 706-106-002

Kurzübersicht für Ziege anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper (Biotin) (ABIN965059)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Meerschweinchen

Wirt: Ziege

Hersteller Produkt- Nr.: 706-106-002

Hersteller: Rockland

Verwendungszweck: F(ab')2 Guinea Pig IgG (H&L) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Produktmerkmale: F(ab')2 Anti-Guinea Pig IgG Alkaline Phosphatase antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Guinea Pig IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Guinea Pig IgG Biotin Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.