Kaninchen anti-Rind (Kuh) IgG (Heavy & Light Chain) Antikörper (PE)

Produktnummer ABIN965020

Hersteller Produkt- Nr.: 701-408-002

Kurzübersicht für Kaninchen anti-Rind (Kuh) IgG (Heavy & Light Chain) Antikörper (PE) (ABIN965020)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Hersteller Produkt- Nr.: 701-408-002

Hersteller: Rockland

Verwendungszweck: F(ab')2 Bovine IgG (H&L) Antibody Phycoerythrin conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Bovine IgG and Bovine Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Produktmerkmale: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Bovine IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.