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Kaninchen anti-Rind (Kuh) IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP))

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP) F(ab')2 fragment
Rockland
Produktnummer ABIN965018
Hersteller Produkt- Nr.: 701-405-002
  • Target Alle IgG Produkte
    IgG
    Fragment
    F(ab')2 fragment
    Bindungsspezifität
    • 2397
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 730
    • 696
    • 598
    • 468
    • 399
    • 240
    • 208
    • 174
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rind (Kuh)
    Wirt
    • 1776
    • 1546
    • 569
    • 261
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Kaninchen
    Klonalität
    • 3406
    • 182
    • 16
    • 1
    Polyklonal
    Konjugat
    • 592
    • 422
    • 422
    • 380
    • 273
    • 262
    • 235
    • 119
    • 106
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 40
    • 39
    • 39
    • 36
    • 36
    • 34
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    Alkaline Phosphatase (AP)
    Applikation
    • 2061
    • 1987
    • 1865
    • 1806
    • 998
    • 920
    • 896
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 58
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Hersteller Produkt- Nr.
    701-405-002
    Hersteller
    Rockland
    Verwendungszweck
    F(ab')2 Bovine IgG (H&L) Antibody Alkaline Phosphatase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
    Produktmerkmale
    F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
    Sterilität
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Bovine IgG whole molecule
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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