Kaninchen anti-Ziege IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN964936

Hersteller Produkt- Nr.: 605-403-b69

Kurzübersicht für Kaninchen anti-Ziege IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed (ABIN964936)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Kaninchen

Hersteller Produkt- Nr.: 605-403-b69

Hersteller: Rockland

Verwendungszweck: Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against to Ms Rt & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Mouse, Rat or Human serum proteins.

Produktmerkmale: Anti-Goat IgG F(ab')2 Rhodamine Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Optional[Immunogen]: Goat IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Goat IgG Pre-Adsorbed Antibody Peroxidase conjugated has been tested by ELISA and is suitable for western blot or dot blot, ELISA and immunohistochemistry, immunoperoxidase electron microscopy and as well as other enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:120,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Goat IgG Antibody Pre-Adsorbed is conjugated to horseradish peroxidase.