Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper (FITC)

Produktnummer ABIN964849

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum.

Produktmerkmale: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Aufreinigung: This product is a F(ab')2  fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Immunogen:

Immunogen: Human IgG whole molecule

Isotyp: IgG

Labeling Ratio: 4.2

Anwendungsinformationen

Applikationshinweise:

Application Note: F(ab')2 Anti-Human IgG (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Centrifuge Tubulin Antibody if not completely clear after standing at room temperature.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store Catalase Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: rabbit F(ab')2 anti-Human IgG Antibody fluorescein Conjugated, rabbit F(ab')2 anti-Human IgG FITC conjugated antibody

Background: F(ab')2 Anti-Human IgG (H&L) Fluorescein Antibody generated in rabbit detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.