Kaninchen anti-Pferd IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN964841

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum.

Aufreinigung: This product is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Immunogen:

Immunogen: Horse IgG whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

ELISA Dilution: 1:20,000-1:100,000

Western Blot Dilution: 1:2,000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Handhabung: Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store Glucose Oxidase Antibody at -20 °C or below prior to opening.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: rabbit F(ab')2 Anti-HORSE IgG Antibody, rabbit Fab2 Anti horse IgG

Background: F(ab')2 Anti-Horse IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.