Kaninchen anti-Meerschweinchen IgG (F(ab')2 Region) Antikörper

Produktnummer ABIN964830

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: F(ab')2 Region

Reaktivität: Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum.

Aufreinigung: This product is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.

Immunogen:

Immunogen: Guinea Pig IgG F(ab')2 fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

ELISA Dilution: 1:20,000-1:100,000

Western Blot Dilution: 1:2,000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Handhabung: Centrifuge product if not completely clear after standing at room temperature.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: rabbit F(ab')2 Anti-GUINEA PIG IgG F(ab')2 fragment Antibody, rabbit F(ab')2 Anti-GUINEA PIG IgG Fab2 fragment Antibody, rabbit Fab2 Anti Guinea Pig IgG Fab2

Background: F(ab')2 Anti-Guinea Pig IgG F(ab')2 Antibody generated in rabbit detects Guinea Pig F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.