Kurzübersicht für Ziege anti-Affe IgA (Free & Bound), (Secretory Component) Antikörper (Biotin) (ABIN784368)
Target
IgA
Klonalität
Polyklonal
Konjugat
Biotin
Applikation
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)
Isotyp
IgG
Bindungsspezifität
Free & Bound, Secretory Component
Reaktivität
Affe
Wirt
Ziege
Produktmerkmale
Molar Ratio: Biotin / IgG aprox. 8.6
Aufreinigung
DEAE-column Chromatography
Immunogen
Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 1 mL sterile distilled water
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Target
IgA
Substanzklasse
Antibody
Hintergrund
Secretory component is present in Monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimmer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.Synonyms: Monkey Immunoglobulin A