Kurzübersicht für Ziege anti-Maus IgG3 Antikörper (HRP) (ABIN783019)
Target
IgG3
Klonalität
Polyklonal
Konjugat
HRP
Applikation
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID)
Isotyp
IgG
Reaktivität
Maus
Wirt
Ziege
Produktmerkmale
Molar Ratio: Peroxidase/IgG aprox. 1.7
Aufreinigung
Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Immunogen
Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 1.0 mL distilled water.
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.