Rekombinanter Kaninchen anti-Human IgD (AA 1-200), (Heavy Chain) Antikörper

Produktnummer ABIN8093253

Kurzübersicht für Rekombinanter Kaninchen anti-Human IgD (AA 1-200), (Heavy Chain) Antikörper (ABIN8093253)

Target: IgD

Antikörpertyp: Recombinant Antibody

Klonalität: Monoklonal

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-701R

Isotyp: IgG, kappa

Produktdetails

Bindungsspezifität: AA 1-200, Heavy Chain

Reaktivität: Human

Wirt: Kaninchen

Verwendungszweck: IgD Heavy Chain Antibody for IHC / IGHD

Immunogen: A recombinant fragment (around amino acids 1-200) of human IGHD protein (exact sequence is proprietary) was used as the immunogen for the IgD Heavy Chain Antibody for IHC.

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the IgD Heavy Chain Antibody for IHC / IGHD Antibody (clone MSVA-701R) should be determined by the researcher.

2. This IGHD/Immunoglobulin heavy constant delta antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: IgD Heavy Chain/IGHD antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Antigendetails

Target: IgD

Substanzklasse: Antibody

Hintergrund: Immunoglobulin D (IgD) is an antibody isotype expressed primarily on the surface of mature naive B lymphocytes and plays an important role in B cell activation and immune regulation. The IgD heavy chain is encoded by the IGHD gene and forms part of the B cell receptor complex together with immunoglobulin light chains. IgD Heavy Chain Antibody for IHC is used to detect this immunoglobulin component in tissue sections, enabling visualization of IgD-expressing B cell populations within lymphoid organs and immune infiltrates.

IgD belongs to the immunoglobulin superfamily and is structurally composed of two delta heavy chains paired with two light chains, forming a monomeric antibody molecule. Membrane-bound IgD functions as an antigen receptor on mature B cells where it cooperates with surface IgM to regulate B cell activation, antigen recognition, and signaling. Engagement of the B cell receptor containing IgD can initiate intracellular signaling cascades that influence lymphocyte survival, differentiation, and antibody production.

In normal human tissues, IgD expression is most prominently detected in B lymphocytes within secondary lymphoid organs such as tonsil, lymph node, and spleen. Within lymphoid follicles, IgD positive B cells are typically enriched in mantle zone regions surrounding germinal centers. This characteristic distribution makes IgD immunohistochemical staining a useful tool for evaluating lymphoid tissue architecture and identifying specific B cell subsets within reactive or neoplastic lymphoid proliferations.

IgD heavy chain antibody detection can also provide diagnostic information in hematopathology. Immunohistochemical staining for IgD may help characterize B cell lineage and maturation stage in certain lymphoid malignancies or plasma cell disorders. In research contexts, detection of IgD contributes to studies of B cell biology, antigen receptor signaling, and the organization of immune cell populations within lymphoid tissues.

A recombinant rabbit monoclonal IgD Heavy Chain Antibody for IHC such as clone MSVA-701R enables sensitive detection of IgD expressing cells in formalin-fixed paraffin-embedded tissue sections. Immunohistochemistry staining typically demonstrates membranous and cytoplasmic localization in B lymphocytes consistent with the distribution of IgD as part of the B cell receptor complex.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P01880