Maus anti-Human IgM (Heavy Chain) Antikörper

Produktnummer ABIN7879450

Kurzübersicht für Maus anti-Human IgM (Heavy Chain) Antikörper (ABIN7879450)

Target: IgM

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM557

Isotyp: IgG1, kappa

Produktdetails

Bindungsspezifität: Heavy Chain

Reaktivität: Human

Wirt: Maus

Verwendungszweck: IgM Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant heavy chain of human IgM was used as the immunogen for this IgM antibody.

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the IgM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

UniProt: P01871