Kurzübersicht für Esel anti-Huhn IgY Antikörper (Agarose Beads) (ABIN7866147)
Target
IgY
Konjugat
Agarose Beads
Applikation
Isotype Control (IsoC)
Isotyp
IgY
Reaktivität
Huhn
Wirt
Esel
Verwendungszweck
Donkey Chicken IgY, conjugated with Agarose
Aufreinigung
Egg-yolk derived IgY Affinity Purified
Immunogen
A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Phosphate buffered saline, pH 7.2 with 0.075 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.