Rekombinanter Maus anti-Human IgG (Fc Region) Antikörper (HRP)

Produktnummer ABIN7581791

Produktdetails

Target: IgG

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Expressionssystem: Phage display

Klonalität: Multiclonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: recombinant Multiclonal anti-human IgG (Fc-specific)

Spezifität: This is an antibody developed by antibody phage display technology using a human naive antibody gene library and human IgG antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.

Keine Kreuzreaktivität: Cat, Rind (Kuh), Hund, Ziege, Pferd, Human, Maus, Kaninchen, Ratte

Kreuzreaktivität (Details): No known cross reactivity. No cross-reactivity to human IgM, human IgE, human IgA, mouse IgG, rabbit IgG, horse IgG, bovine IgG, goat IgG, rat IgG, dog IgG, cat IgG. Other species were not tested.

Produktmerkmale: Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all four different subclasses of human IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.

Aufreinigung: Protein A purification

Güteklasse: Animal-Free

Immunogen: No immunization, animal-free antibody development. Antigen: human intravenous immunoglobulins (IVIg)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:1,000 - 1:20,000 ELISA: 1:1,000 - 1:50,000 Optimal working dilution should be determined by the investigator

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, 0,02 % ProClin300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Molekulargewicht: 150 kDa

UniProt: P01857