Rekombinanter Maus anti-Ziege IgG Antikörper (Alexa Fluor 488)

Produktnummer ABIN7581790

Kurzübersicht für Rekombinanter Maus anti-Ziege IgG Antikörper (Alexa Fluor 488) (ABIN7581790)

Target: IgG

Antikörpertyp: Recombinant Antibody

Klonalität: Multiclonal

Konjugat: Alexa Fluor 488

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Animal-Free

Isotyp: IgG2a

Produktdetails

Reaktivität: Ziege

Wirt: Maus

Expressionssystem: Phage display

Verwendungszweck: recombinant Multiclonal anti-goat IgG

Spezifität: This is an antibody developed by antibody phage display technology using a human naive antibody gene library and goat IgG antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.

Keine Kreuzreaktivität: Ziege, Human, Maus

Kreuzreaktivität (Details): No known cross reactivity. No cross reactivtiy to mouse IgG, human IgG, goat IgG. Other species were not tested.

Produktmerkmale: Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all subclasses of goat IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.

Aufreinigung: Protein A purification

Immunogen: No immunization, animal-free antibody development.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:50 - 1:1,000 ELISA: 1:50 - 1:1,000 IHC: 1:50 - 1:1,000 Optimal working dilution should be determined by the investigator

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4,

Lagerung: -20 °C

Antigendetails

Target: IgG

Substanzklasse: Antibody

Molekulargewicht: 150 kDa