Kaninchen anti-Llama VHH Antikörper

Produktnummer ABIN7565718

Kurzübersicht für Kaninchen anti-Llama VHH Antikörper (ABIN7565718)

Target: VHH

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Llama

Wirt: Kaninchen

Verwendungszweck: Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies.

Kreuzreaktivität (Details): Anti-VHH is directed against the llama VHH protein fragment.

Produktmerkmale: Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies. The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.

Aufreinigung: The product was purified from monospecific antiserum by protein A chromatography. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-VHH antibody has been tested by ELISA and Western blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed. Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:10,000-1:50,000 Other: 1.0 mg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: VHH

Hintergrund: The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.