Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN7539582

Hersteller Produkt- Nr.: 609-1302-1.0

Kurzübersicht für Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN7539582)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Güteklasse: High-quality product from Rockland

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 609-1302-1.0

Hersteller: Rockland

Verwendungszweck: Goat Anti-Human IgG H&L (HRP)

Spezifität: IgG (H&L)

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.

Immunogen: Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.
Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Anti-Human IgG (H&L) peroxidase conjugated antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Jian, Xiujian, Yuangang, Yan, Lianchao, Duthie, Yan: "Evaluation of antibody detection against the NDO-BSA, LID-1 and NDO-LID antigens as confirmatory tests to support the diagnosis of leprosy in Yunnan province, southwest China." in: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 114, Issue 3, pp. 193-199, (2021) (PubMed).

Phan, Subramanian, Kim, Murphy, Pettie, Carter, Anishchenko, Barrett, Craig, Tillery, Shek, Harrington, Koelle, Wald, Veesler, King, Boonyaratanakornkit, Isoherranen, Greninger, Jerome, Chu, Staker et al.: "In silico detection of SARS-CoV-2 specific B-cell epitopes and validation in ELISA for serological diagnosis of COVID-19. ..." in: Scientific reports, Vol. 11, Issue 1, pp. 4290, (2021) (PubMed).

Huang, Tan, Chen, Huang, Harvey, Hussain, Chen, Harding, Gilbert-Jaramillo, Liu, Knight, Schimanski, Shih, Lin, Cheng, Cheng, Huang, Lin, Jan, Ma, James, Daniels, McCauley, Rijal, Townsend: "Breadth and function of antibody response to acute SARS-CoV-2 infection in humans." in: PLoS pathogens, Vol. 17, Issue 2, pp. e1009352, (2021) (PubMed).

Serrano-Coll, Muñoz, Camilo Beltrán, Duthie, Cardona-Castro: "Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibodies as indicators of leprosy reactions and neuritis." in: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 111, Issue 3, pp. 125-131, (2018) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1