Rekombinanter Alpaca anti-Kaninchen IgG Antikörper (HRP)

Produktnummer ABIN7272954

Kurzübersicht für Rekombinanter Alpaca anti-Kaninchen IgG Antikörper (HRP) (ABIN7272954)

Target: IgG

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Klonalität: Monoklonal

Konjugat: HRP

Applikation: Western Blotting (WB)

Klon: 10E10

Produktdetails

Reaktivität: Kaninchen

Wirt: Alpaca

Expressionssystem: E.coli

Spezifität: Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

Aufreinigung: Produced in: E.coli

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute HRP-conjugated sdAbs with 200 µL pure water.

Buffer: lyophilized from HRP-Protector.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: The immune repertoire of rabbits, like of many other mammals, consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source for polyclonal antibodies, which were employed in various fields of life sciences. They are employed either directly from animal sera, or as affinity purified reagents. In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our single domain antibodies (sdAbs, or Nanobodies) bind the constant domain (Fc) of any rabbit IgG specifically and with high affinity, making this secondary tool ideal for demanding imaging applications.