Rekombinanter Alpaca anti-Maus IgG1 Antikörper (Biotin)

Produktnummer ABIN7272944

Kurzübersicht für Rekombinanter Alpaca anti-Maus IgG1 Antikörper (Biotin) (ABIN7272944)

Target: IgG1

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 10A4

Produktdetails

Reaktivität: Maus

Wirt: Alpaca

Expressionssystem: E.coli

Spezifität: Mouse IgG1, does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Aufreinigung: Produced in: E.coli

Immunogen: sdAb anti-Mouse is a species-specific sdAb directed against mouse IgG1. anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: IgG1

Substanzklasse: Antibody

Hintergrund: Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either lambda or kappa light chain.