Rekombinanter Alpaca anti-Huhn IgY Antikörper (Biotin)

Produktnummer ABIN7272937

Kurzübersicht für Rekombinanter Alpaca anti-Huhn IgY Antikörper (Biotin) (ABIN7272937)

Target: IgY

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 3G2

Produktdetails

Reaktivität: Huhn

Wirt: Alpaca

Expressionssystem: E.coli

Spezifität: Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

Aufreinigung: Produced in: E.coli

Immunogen: sdAbs anti-chicken IgY binds strongly and specifically to all IgYs. The anti-Chicken IgY sdAb is a single-domain anitbody against Chicken IgY, that can be labeled or immobilized by the customer.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: IgY

Substanzklasse: Antibody

Hintergrund: The immunorepertoire of chickens is much simpler compared to any other mammal, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.