Rekombinanter Alpaca anti-Kaninchen IgG Antikörper (Alexa Fluor 647)

Produktnummer ABIN7272930

Kurzübersicht für Rekombinanter Alpaca anti-Kaninchen IgG Antikörper (Alexa Fluor 647) (ABIN7272930)

Target: IgG

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Klonalität: Monoklonal

Konjugat: Alexa Fluor 647

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 10E10

Produktdetails

Reaktivität: Kaninchen

Wirt: Alpaca

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-Rabbit IgG Alexa Fluor™ 647, Clone(s) 10E10

Spezifität: Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

Aufreinigung: Produced in: E.coli

Immunogen: This camelid sdAB anti-Rabbit IgG is a species-specific antibody directed against IgGs from Rabbit. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Like many other mammals, the immune repertoire of rabbits consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source of polyclonal antibodies, which were employed in various fields of life sciences. They are used either directly from animal sera, or as affinity-purified reagents. In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our alpaca-derived single domain antibody (sdAb, or nanobody) binds the constant domain (Fc) of any rabbit IgG, specifically and strongly, which makes this secondary tool ideal for demanding imaging applications.