Rekombinanter Alpaca anti-Huhn IgY Antikörper (Atto 488)

Produktnummer ABIN7272913

Kurzübersicht für Rekombinanter Alpaca anti-Huhn IgY Antikörper (Atto 488) (ABIN7272913)

Target: IgY

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Klonalität: Monoklonal

Konjugat: Atto 488

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Klon: 3G2

Produktdetails

Reaktivität: Huhn

Wirt: Alpaca

Expressionssystem: E.coli

Spezifität: Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

Aufreinigung: Produced in: E.coli

Immunogen: anti-Chicken is a species-specific camelid sdAB directed against immunoglobulins from Chicken.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: IgY

Substanzklasse: Antibody

Hintergrund: The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.