Ziege anti-Ratte IgG Antikörper (DyLight 649)

Produktnummer ABIN6699155

Hersteller Produkt- Nr.: 612-143-002

Kurzübersicht für Ziege anti-Ratte IgG Antikörper (DyLight 649) (ABIN6699155)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 649

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Ratte

Wirt: Ziege

Hersteller Produkt- Nr.: 612-143-002

Hersteller: Rockland

Verwendungszweck: Rat IgG (H&L) Antibody Dylight™ 649 Conjugated

Produktmerkmale: Goat Anti-Rat IgG DyLight 649™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649™ Conjugation,

Immunogen: Rat IgG, whole molecule

Labeling Ratio: 2.9

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Rat IgG DyLight™ 649 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Riedemann, Sutor, Bergami, Riedemann: "Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line." in: The Journal of comparative neurology, Vol. 527, Issue 14, pp. 2215-2232, (2020) (PubMed).

Riedemann, Straub, Sutor: "Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200567, (2019) (PubMed).

Riedemann, Schmitz, Sutor: "Immunocytochemical heterogeneity of somatostatin-expressing GABAergic interneurons in layers II and III of the mouse cingulate cortex: A combined immunofluorescence/design-based stereologic study." in: The Journal of comparative neurology, Vol. 524, Issue 11, pp. 2281-99, (2017) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody