Ziege anti-Ratte IgG Antikörper (DyLight 549) - Preadsorbed

Produktnummer ABIN6699152

Hersteller Produkt- Nr.: 612-142-120

Kurzübersicht für Ziege anti-Ratte IgG Antikörper (DyLight 549) - Preadsorbed (ABIN6699152)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 549

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Ratte

Wirt: Ziege

Hersteller Produkt- Nr.: 612-142-120

Hersteller: Rockland

Verwendungszweck: Rat IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins

Produktmerkmale: Goat Anti-Rat IgG DyLight 549™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 549™ Conjugation,Anti-Rat IgG (H&L) DyLight™549 Antibody generated in goat detects reactivity to Rat IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Rat IgG whole molecule

Labeling Ratio: 6.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rat IgG (H&L) DyLight™549 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

van den Berg, East, Mitra, Jansen: "p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A." in: Molecular biology of the cell, Vol. 34, Issue 5, pp. br6, (2023) (PubMed).

Ayyar, Ettayebi, Salmen, Karandikar, Neill, Tenge, Crawford, Bieberich, Prasad, Atmar, Estes: "CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection." in: Nature communications, Vol. 14, Issue 1, pp. 1148, (2023) (PubMed).

Yamamoto, Suwa, Sugiyama, Okashita, Kawaguchi, Tani, Matsubara, Nakamura, Seki: "The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency." in: Journal of cell science, Vol. 133, Issue 15, (2021) (PubMed).

Ide, Mochiji, Ueki, Yamaguchi, Shigenobu, Hirono, Wakabayashi: "Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii." in: Biochemistry and biophysics reports, Vol. 7, pp. 379-385, (2016) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to DyLight™549.