Ziege anti-Kaninchen IgG (Fc Region) Antikörper (DyLight 800)

Produktnummer ABIN6699130

Hersteller Produkt- Nr.: 611-145-003

Kurzübersicht für Ziege anti-Kaninchen IgG (Fc Region) Antikörper (DyLight 800) (ABIN6699130)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 611-145-003

Hersteller: Rockland

Verwendungszweck: Rabbit IgG Fc Antibody DyLight™ 800 Conjugated

Produktmerkmale: Goat Anti Rabbit IgG F(c) DyLight 800™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 800™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 800™ conjugated,Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Immunogen: Rabbit IgG F(c) fragment

Labeling Ratio: 2.2

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG F(c) DyLight ™ 800 conjugate has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Joshi, Almgren-Bell, Anaya, Todd, Van Dyken, Seth, McIntire, Singamaneni, Sutterwala, Morley: "L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis." in: Cell reports, Vol. 38, Issue 11, pp. 110507, (2022) (PubMed).

Fang, Xu, Liu, Cao, Qiu, Peng: "Interleukin 17A deficiency alleviates neuroinflammation and cognitive impairment in an experimental model of diabetic encephalopathy." in: Neural regeneration research, Vol. 17, Issue 12, pp. 2771-2777, (2022) (PubMed).

Anderson, Sastalla, Earland, Mahnaz, Moore, Otaizo-Carrasquero, Myers, Myles, Datta, Myles: "Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632." in: PLoS ONE, Vol. 13, Issue 9, pp. e0198862, (2019) (PubMed).

Onischenko, Tang, Andersen, Knockenhauer, Vallotton, Derrer, Kralt, Mugler, Chan, Schwartz, Weis: "Natively Unfolded FG Repeats Stabilize the Structure of the Nuclear Pore Complex." in: Cell, Vol. 171, Issue 4, pp. 904-917.e19, (2017) (PubMed).

Kohnz, Roberts, DeTomaso, Bideyan, Yan, Bandyopadhyay, Goga, Yosef, Nomura: "Protein Sialylation Regulates a Gene Expression Signature that Promotes Breast Cancer Cell Pathogenicity." in: ACS chemical biology, Vol. 11, Issue 8, pp. 2131-9, (2017) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.