Schaf anti-Kaninchen IgG Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6699121

Hersteller Produkt- Nr.: 611-645-122

Kurzübersicht für Schaf anti-Kaninchen IgG Antikörper (DyLight 800) - Preadsorbed (ABIN6699121)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Schaf

Hersteller Produkt- Nr.: 611-645-122

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins

Produktmerkmale: Sheep Anti Rabbit IgG Antibody DyLight 800™ Conjugate, Sheep Anti-Rabbit IgG DyLight 800™ Conjugated Antibody,Anti-Rabbit IgG Antibody DyLight™800 generated in sheep detects rabbit IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 2.5

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Arroum, Borowski, Marx, Schmelter, Scholz, Psathaki, Hippler, Enriquez, Busch: "Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation." in: Biological chemistry, Vol. 404, Issue 5, pp. 399-415, (2023) (PubMed).

Joshi, Almgren-Bell, Anaya, Todd, Van Dyken, Seth, McIntire, Singamaneni, Sutterwala, Morley: "L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis." in: Cell reports, Vol. 38, Issue 11, pp. 110507, (2022) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Lan, Yuan, Lin: "Sulforaphane induces p53‑deficient SW480 cell apoptosis via the ROS‑MAPK signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7796-7804, (2018) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.