Esel anti-Kaninchen IgG Antikörper (DyLight 680) - Preadsorbed

Produktnummer ABIN6699117

Hersteller Produkt- Nr.: 611-744-127

Kurzübersicht für Esel anti-Kaninchen IgG Antikörper (DyLight 680) - Preadsorbed (ABIN6699117)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 680

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Esel

Hersteller Produkt- Nr.: 611-744-127

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Produktmerkmale: Donkey Anti-Rabbit IgG Antibody DyLight 680™ Conjugated, Donkey Anti Rabbit IgG DyLight 680™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 680 Antibody generated in donkey detects reactivity to Rabbit IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 2.8

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG (H&L) DyLight 680 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

van den Berg, East, Mitra, Jansen: "p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A." in: Molecular biology of the cell, Vol. 34, Issue 5, pp. br6, (2023) (PubMed).

Conway, Rockhold, SantaCruz-Calvo, Zukowski, Pugh, Hasturk, Kern, Nikolajczyk, Bharath: "Obesity and Fatty Acids Promote Mitochondrial Translocation of STAT3 Through ROS-Dependent Mechanisms." in: Frontiers in aging, Vol. 3, pp. 924003, (2022) (PubMed).

Tucker, Alicea Pauneto, Barnett, Coleman: "Chronic Ethanol Causes Persistent Increases in Alzheimer's Tau Pathology in Female 3xTg-AD Mice: A Potential Role for Lysosomal Impairment." in: Frontiers in behavioral neuroscience, Vol. 16, pp. 886634, (2022) (PubMed).

Mendenhall, Liu, Portley, O'Mard, Fattah, Szabo, Bugge, Khillan, Leppla, Moayeri: "Anthrax lethal factor cleaves regulatory subunits of phosphoinositide-3 kinase to contribute to toxin lethality." in: Nature microbiology, Vol. 5, Issue 12, pp. 1464-1471, (2021) (PubMed).

Coleman, Zou, Qin, Crews: "HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism." in: Brain, behavior, and immunity, Vol. 72, pp. 61-77, (2019) (PubMed).

Lin, Chen, Wang, Cai: "Emodin promotes the arrest of human lymphoma Raji cell proliferation through the UHRF1‑DNMT3A‑∆Np73 pathways." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 6544-6551, (2018) (PubMed).

Coleman, Maile, Jones, Cairns, Crews: "HMGB1/IL-1β complexes in plasma microvesicles modulate immune responses to burn injury." in: PLoS ONE, Vol. 13, Issue 3, pp. e0195335, (2018) (PubMed).

Chen, Zhang, Xu, Wang, Shi, Xu, Zhang, Wang, Li: "HOXC6 promotes gastric cancer cell invasion by upregulating the expression of MMP9." in: Molecular medicine reports, Vol. 14, Issue 4, pp. 3261-8, (2017) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.